"Recombinant DNA methodology led to the development of DNA or gene cloning. By the end of the 1970s, the method of inserting short DNA fragments into a plasmid or viral chromosome, and then replicating them in bacterial or eukaryotic dead pigs was relatively mature. After that, I will elaborate on how to implement gene cloning and the reasons for the breakthrough changes in molecular biology caused by this technology."

Yang Rui said this and paused for a moment, and said: "A paper I did before, which was designed based on this study to study the structure of potassium ion channels by cloning mutant genes."

The experts and professors who were originally ready to move were all silent.

The publication of domestic scholars in "CELL" is extremely rare nationwide. Yang Rui's paper on potassium ion channel has been known to everyone in the domestic biology community.

If such a paper is left to a middle-aged scholar, it will be worshipped as a legend.

Throwing it to an academician is equivalent to re-branding a layer of golden body.

Yang Rui wrote this article "CELL", which naturally had a stronger impact. However, Yang Rui's identity slightly delayed its power, allowing it to explode, but slowly spread its power.

However, the classroom has always been a place where teachers can fully open their power.

This is true for professors in the classroom, and so is part-time teachers in the classroom.

At this time, Yang Rui's paper is enough to shock all monsters and monsters.

At the same time, the academic difficulty of potassium ion channel papers, as a high-end application of DNA recombination technology, is itself unsatisfactory.

The teachers who originally wanted to speak now all lowered their heads and took notes obediently.

They are all people who have read Yang Rui's papers, and some people even know about the scientific research competition between Yang Rui and Richard, so no one wants to question Yang Rui in terms of DNA recombination technology.

An CELL has hundreds or thousands of words, and at most a single digit icon is added. The information contained in it is something that ordinary scholars cannot understand in their lifetime. When someone is the first to achieve the results, ordinary scholars can only worship it.

Scholars have been immersed in it all year round and can fully understand the difficulty of DNA recombination technology, and the application of potassium ion channels to DNA recombination technology is top-notch.

If Yang Rui doesn't mention this, everyone can choose to ignore it. When Yang Rui put it forward, compared with what he said before, no matter how strong a person is, he must admit in his heart: Yang Rui is qualified to say that classic genetics methods are behind.

Scholars take notes while thinking, focusing on the enemy.

The atmosphere in the classroom is getting more and more solemn every minute.

Although the students couldn't hear the utmost, they were all so arrogant that they were afraid to speak out by the people around them.

"Simply put, from the 1960s to the 1980s, the genetic field has gone through two stages, which is what I mentioned earlier, the era of classical genetic theory and the era of recombinant DNA technology. But today I will introduce to you the third era that is the era of PCR!"

In a trance of everyone, Yang Rui continued: "The full name of PCR is polymerase chain reaction. I wrote the English words on the blackboard. This is a polymerase reaction I invented myself. It has been recognized and supported by colleagues from all over the world. As of last week, my paper on this technology has exceeded 2,000 citations..."

Yang Rui felt very good when he said this: I created an era of biology, that's it!

If you don’t agree, come and argue!

No one dares to argue.

All the experts, young or old teachers, whether looking up and bowing their heads, bowed their heads again in a brief shock, surprise and understanding.

What does it mean if the number of citations exceeds 2000?

The influencing factor of a journal is the total number of citations of articles from the previous two years, and the total number of articles from the previous two years. In other words, the influencing factor is the average number of citations of the article.

A journal with an impact factor of 1.0 means that the average number of cited papers in this journal has been 1 in the past two years.

In the SCI index, the impact factor 1.0 is not the bottom line, there are many journals with the impact factor 0.5, and these journals are all good journals in terms of the journal world.

As for what are the influencing factors that make people fascinated by the top journal CNS? Generally speaking, they are more than 30 or more, and they are less than 40.

To convert, under normal circumstances, papers published in Cells, Nature and Science have an average citation number of more than 30 and less than 40.

What is the case in which it is not the usual situation? It is the case where Yang Rui’s paper is released.

As a technology that is destined to rewrite the history of biology and the history of human science, it is so-called reasonable and unexpected to obtain 2,000 citations in just a few months.

And its value in citations is now equivalent to 50 CNS-level papers, 2,000 or even 4,000 ordinary SCI-level papers.

Compared with similar excellent super-top papers, PCR is also a record-breaking excellence.

Among the super-top papers published in Nature, the higher citation numbers are generally 200 to 300. Few heard of 400 or even 500 citation numbers. In the field of science, this is no longer a simple double-double problem. There are only ones in the world of biology laboratories. Everyone has their own fields. It is not a theory that involves a very wide range of citations. If it reaches 100, it is already considered popular all over the world.

But the end point of PCR is far from 2,000 citations.

This is a technology destined to rewrite history, and the global biological laboratories are about to understand it, use it, and quote it regardless of the field.

Yang Rui stood on the podium with great confidence, facing the "squeaking" camera, waiting for someone to come to debate with him.

He waited for a full minute.

For a long minute, the classroom was silent.

"Does no one have any objections?" Yang Rui's clear voice echoed in the classroom.

With the humility of the Chinese, Yang Rui ranked his PCR technology alongside classic genetic theory and recombinant DNA technology, calling it the third generation technology, which should have been called "arrogant".

However, after a minute, no one did.

Yang Rui waited for another minute, but no one did it.

Yang Rui sighed regretfully and even sighed sadly.

Although 2,000 citations are enough to crush any academic enemy, it is still a bit lonely to drive straight into the world without an enemy.

Loneliness seeks defeat? Not even.

After all, this is a lecture hall, and the lecturers can express their views and cognition at will, and it has no authority.

If it was an international conference, the person raising his hands would probably break through the sky.

However, although the lecture hall does not have the solemnity of an international conference, it is still a formal occasion.

Yang Rui is more willing to appear in front of the world in a more comprehensive manner.

Writing books and readings, Lishu needs such a thick message as a book to prove himself.

"Since there is no objection, I will use this as a basis to talk about research methods specifically for genomes." Yang Rui turned around and wrote the words "Genome Research Methods" on the blackboard, which instantly detonated the audience.

Compared to the banner Yang Rui had put up for PCR, the direct technical guidance obviously made scholars more excited.

Of course, the students didn't understand what the experts and professors around them were so excited that they looked at the blackboard in a daze.

Finally, Wang Dongming, who has always been bold and conceited, could no longer hold back and raised his hand and said, "Teacher Yang, where can we see the textbook of this class?"
Chapter completed!