Chapter 1061 The distance between the two eggs
Gu Qiang followed the plan and first did estrus synchronously.
Synchronous estrus means using certain exogenous hormones to artificially control the estrus cycle of a group of female animals, so as to organize embryo transfer in a planned manner within a predetermined time.
There are many drugs that can be used for exogenous hormones, with strong and weak control effects, high and low efficiency, which is not very rare. What Yang Rui discussed with Professor Wang Yong that day was that the existing exogenous hormones were easier to use ready-made naturally than redeveloped, let alone this non-critical part of mature drugs.
There were 186 of the 200 Simmental cows on the scene, and 186 of them entered the estrus stage. It was calculated that the success rate of more than 90%, so it would not be easy to improve it any more.
The remaining 14 heads looked at their companions at a loss, wondering why they suddenly became different.
A younger cow was even more anxiously spinning around.
She was surrounded by cows in heat, mooing constantly, bent her waist, and was impatient, and it looked different from usual.
After turning the 20th or 25th circle, this very young cow made a decision resolutely. She also bent her waist and gave up the feed in the trough as other cows. Then she kept raising her hooves in an impatient manner and turned her head to the bull's cowshed where the bull was.
Finally, the heifer successfully attracted the attention of a veterinarian.
He put on his heavy rain boots again, took the utensils, and went up to check.
Gu Qiang also looked up and asked, "Is he in heat? That's 187?"
Comrade Qin Yue, a veterinarian from China Academy of Pastoral, looked in the cattle for a while, came back and took off his glasses and wiped them, saying, "No, it's still 186."
"No, right? It looks like it."
"It's pretending." Qin Yue put on glasses and answered concisely.
The crowd surrounding the cow pen let out a soft laugh.
Gu Qiang shook his head in surprise.
He stayed in the laboratory for a long time and stayed at the site for a short time. However, most researchers are like this. Those sent to the site are often the lowest-graded members of the research team or project team, as assistant researchers, doctoral students, graduate students, and sometimes even undergraduates.
However, genetic engineering laboratories do not have low-age experimental dogs for exploitation.
In the genetic engineering laboratory, Yang Rui has the lowest seniority. He has only three years of scientific research experience, which is similar to an ordinary master's graduate. In the research institute, he is equivalent to an assistant researcher qualification - so Gu Qiang had to take the initiative himself.
Fortunately, China Animal Husbandry Corporation will provide complete assistance and manpower for the sake of 200 Simmental cattle.
Just after Gu Qiang shouted "The number of ovulations began", a cattle herder led the cows in heat one by one, first weighing them, then making records.
Four veterinarians from the Chinese herdsmen lined up and gave the cows injections in turn.
Qin Yue was at the forefront, responsible for both the injections and the number reporting and judging the health of the cow.
Gu Qiang stood behind the veterinarians, and according to the reported weight data, he sucked gonadotropin in the syringe, and handed it to the veterinarians separately.
Yu Xing'an was helping Gu Qiang. He stared at the veterinarians and ensured that all the medicines in the syringe were injected into the correct cow.
When the entire injection process was completed, Yu Xing'an even poured a large amount of foamy water on the veterinarians in the name of disinfection.
Even if anyone squeezes the potion a little secretly on his body, the whole bucket of disinfectant will destroy the activity of the potion.
Director Tie from Qingmu has been reporting his duties in the capital for some time recently. Today, he came over here specially. Seeing this scene, he couldn't help but smile and said to Director Mei, who was proud of him, "Look, no matter how much money you are from Zhongmu, you still don't trust."
"It's not necessary to provoke and disagree. You're too young to play with this." The proud Director Mei has always despised the strategic ability of Director Tie, who has wide shoulders and strong body.
Director Tie smiled and said, "Don't be sowed, look, there's just a gonadotropin, they're all hiding fear you know."
Director Mei's face was a little unfair and said, "It's normal for them to develop some special hormones that they won't let other units know about it."
"So what if you can get five ovulations at a time? I heard that the latest foreign technology is all about gonadotropins that can ovulate six or seven ovulations. This thing is not used much. At worst, you can just spend money to buy it. You still have less money?" Director Tie didn't care about the relationship with the Haidian District Genetic Engineering Laboratory and the Sino-Academic Corporation. He just wanted to disgust Comrade Lao Mei. Of course, if the relationship between the two parties is bad, it would be even better. He dreamed of monopolizing this resource.
Director Mei has been inconsistent with Director Tie since he went to school, but he secretly agreed with what he said just now.
Director Mei twisted his neck uncomfortable and said, "Yang Rui is a young man after all. This Haidian District Genetic Engineering Laboratory is also a new laboratory. They have finally developed new technologies. Even if it only has 60% of the performance of foreign products, it is worthy of respect."
"Hey, those who work in Beijing are different. They are spoken in a string of official language." Director Tie laughed twice, but shouted to the veterinarian in front of him: "Do you see eggs? How many eggs?"
What he asked was naturally how many ovulations were released.
Anyone who has studied junior high school biology knows that embryos come from the combination of a sperm and an egg. The number of sperm released by a bull is extremely large at one time, and it can be said that there are thousands of eggs, and eggs are extremely scarce.
For cows with only one birth, the number of eggs in cows is smaller, often only three to four. In other words, the number of embryos that can be transferred for embryos is actually determined by the number of eggs.
If a cow ovulates 4 at a time and all develops into Class A embryos, then after one embryo segmentation, it is possible to obtain 8 half embryos.
Even if the success rate of half-embryo transplantation is 50%, for good-bred cattle, this is also a double breeding efficiency, not to mention that multiple ovulations can be performed within one year.
However, commercial operations have never been able to achieve ideal data, and the desire for capital cannot be met with 4 times or 8 times efficiency.
In developed countries outside China, the reason why biotech companies are tirelessly spending money on embryo transfer technology is that they have increased their success rate from all aspects.
For example, during the embryo collection process, the purpose of injecting gonadotropin is to obtain more than 4 eggs...
In an era when an egg basically represents a cow, it is inevitable to develop better and better gonadotropins.
From the 1970s to the 1980s, the change of gonadotropin was not only to obtain more eggs, but also to reduce side effects and increase the proportion of available embryos.
In this subdivided field, domestic research institutes naturally lag behind again. It should be said that there is basically no research, so they introduced and used technologies introduced from Eastern Europe in the 1950s and 1960s.
However, the difference between five eggs and seven eggs is not one man, but two cows.
Veterinarian Qin Yue, wearing rain boots and holding a record book, walked over, shouted "Director Mei", then called "Director Tie", and then asked softly: "You call me?"
"I'm calling you, Director Mei wants to know how many eggs a cow has?" Director Tie's face was not visible, and he looked very serious.
"I don't know it very well now." Qin Yue looked at the notebook and hesitated a little.
"Just say you know." Director Tie has been a ranch for his whole life, leaving no room for speaking.
Qin Yue turned his face to Director Mei.
Director Mei said "hmm" and said, "Just ask, just say as much as you know."
"Yes." Qin Yue looked down at the notebook again and said, "We have checked the twelve cows. The corresponding ovulation numbers are 10, 12, 11, 10, 13, 9, and 12..."
"Wait, what you are asking about is the number of eggs and how many cows are there?" Director Tie glared.
Chapter completed!