Science was originally a human activity formed by summary.

I lit a bonfire at night, and some insects hit the fire, picked it up and chewed it, and it was crispy. So I wondered, why do some insects hit the fire stupidly? Is they stupid? Or? Is it because a god knows that I am hungry, so he gave me the insects to eat?

After a long summary, the insect-eating person finally came to a conclusion, because no matter how I do it, the insect will hit the fire. Therefore, it is obviously not because of God, so the insect is stupid.

Later, people discovered that many bugs were navigating through moonlight, so they misdirected to the light source, which once again confirmed that they were foolish.

After three steps, conjecture, summary, and confirmation, the process of finally drawing conclusions is how most scientific research is carried out.

G protein-coupled receptors are very high-end, but the research process is actually the same.

It’s like climbing the Himalayas requires all kinds of equipment and preparations, but in the final analysis, there is no essential difference between climbing Mount Tai or the small hill bag in the next village.

However, there are still high-end reasons for high-end.

For example, G protein-coupled receptors are now stuck in the first step.

guess.

Yes, just like humans don’t understand Mars, they can only guess what is above, what is underground, and what is composed of it. G-protein coupled receptors are also in complex speculation for humans.

People have put forward various conjectures, and they don’t know if they have missed 1 million possible correct conjectures.

So, how to solve this problem?

Just like ancient humans, we observe.

More, more, more observations...

Before bovine rhodopsin protein, humans actually didn’t even have the chance to observe.

Because the G protein-coupled receptor is very unstable, difficult to extract, difficult to isolate, difficult to purify, and even more difficult to observe...

Things in the microscopic world are so troublesome. You can't use tweezers to pinch it out. Even if you have such superpowers, you don't know which one you need to pinch out? Because you can't recognize it.

For nanoscale proteins, the only way to observe is to make it into a solution, continuously dilute it, and then draw a small amount of solution for observation.

For example, dissolve 2 grams of protein powder in 100 ml of liquid... However, the aggregation problem of proteins needs to be solved, and for G protein coupled receptors, the more troublesome part is still to come.

This product is unstable and it will collapse in solution.

Think about it, a single scientific research dog gave up the Mid-Autumn Festival, did not have Christmas Eve, forgot Christmas, and could not celebrate the Spring Festival. Finally, on February 14, at the hand speed that he had been practicing for many years, he shot them under the microscope before the furnace of warm G protein-coupled receptors could react, and then...

They actually collapsed.

Find the despair volume of a single scientific research dog at this time - knocking on the blackboard, drawing the key points, and finding the shadow area is useless. That is an exercise for junior high school students. The shadow area is the projection area of ​​an object. Unless the object is regular, the projection area cannot be used to calculate the volume.

As for the best reference for the volume of an irregular body such as despair, the best reference is Archimedes:

Put an object into water, and the volume of water discharged is the volume of the object.

But what if despair dissolves in water? What if despair collapses in solution? Why can't I find a solution that can not react with despair?

So desperate!!

In short, before bovine rhodopsin protein, human observation of G protein-coupled receptors was like this vicious cycle.

Even observation is a vicious cycle, and even a step of guessing cannot be fully carried out. It can be imagined that the subsequent summary and confirmation are like a blind man touching an elephant.

However, the result of a bit fragmented is better than nothing.

Even with such fragmented results, the Nobel Prize was given without hesitation, and several more were given.

Wait until the bovine rhodopsin protein emerges.

The bigwigs in the biological world finally breathed a sigh of relief: Finally there are enough moths, we can have a good meal... No, we can observe it carefully.

However, Dana is Dana after all.

Big people are never satisfied.

Therefore, there are always laboratories who can't help but think that it doesn't feel enough of a moth, will it be monotonous if you eat it every day, and what if you get constipated?

In addition, it is only an observation stage, so everyone does not know how large the sample is needed to observe, so it has not yet entered the white-hot number-pronged stage.

In fact, what you need to enter is to look at the number of proteins you spelled.

If some laboratories find better observation objects or more observation objects, they will be able to achieve twice the result with half the effort in the following scientific research competition.

However, Yang Rui knew very well that they were thinking too much.

Bovine rhodopsin protein is the best.

To some extent, it is unique.

The remaining few left, or the varieties found next, are either too expensive, not easy to use, or the sources are not wide enough. It doesn’t matter if you change to another scientific research project and use more expensive varieties or less numerous varieties. Anyway, there is enough funding, and it will be over once you have to endure it.

Unfortunately, the three-dimensional mechanism of G protein-coupled receptors is really lucky.

I want to buy 5 million for 2 yuan. This kind of thing is good as an entertainment project, but it would be too much to try my luck when I expand my goal to 20 million US dollars.

Therefore, in the conjecture stage, sufficient observation is essential.

From the beginning, Yang Rui was the most familiar with the project and the strongest comprehensive strength, and sent Su Xiankai to make beef rhodopsin protein, because he knew what the core of the problem was.

As an active calf and a big bull in later generations, Su Xiankai's creativity was quickly inspired.

Various dry powder culture media, trypsin, bovine serum, and fresh cow eyes were sent to the Peking University ion channel laboratory, and passed through a low-temperature ultracentrifuge, an automatic gel chromatograph, a protein analyzer... and then sent to the tunnel microscope for observation. The whole process was decomposed step by step by step by Su Xiankai.

This is true for any ecology behavior, constantly decomposing steps, constantly improving proficiency, and then continuously increasing yield.

At the same time, as a laboratory and a researcher, Su Xiankai was not satisfied with simple production.

Simplifying the steps and even developing a better way of production is what he insists on doing.

Yang Rui was also quite impressed by this.

If another researcher is in the same position as Su Xiankai, he might have just accepted his fate and started a simple production. Perhaps in just a few years, such a researcher will become a simple research worker.

But Su Xiankai was not like this.

He worked hard to study, starting from figuring out each production step, and then doing optimization step by step. And these tasks were not copied directly from the book, so Su Xiankai also did a great job.

The production of bovine phyroxine protein also doubled.

2 times.

4 times.

8 times.

Compared with the initial production capacity, Su Xiankai has increased it unexpectedly to ten times.

This number is far beyond that of first-class foreign laboratories.

Yang Rui also quietly breathed a sigh of relief.

A comprehensive catch-up is never done in an instant, but as long as the basic indicators catch up, no matter how weak the subsequent indicators are, they will not be much weaker.

Of course, just catching up with first-class foreign laboratories is not Yang Rui's goal.

At the meeting that week, Yang Rui made another request and wrote it directly on the blackboard in the conference room: "Congratulations to the production of the bovine phyropin protein group for setting a new high. Please continue to work hard and increase the output tenfold!"

Su Xiankai was busy until the end before entering the conference room. The moment he saw the handwriting on the blackboard, he lost the courage to breathe.

"You just strangle me to death." Su Xiankai lay down on the chair, not wanting to move anymore.
Chapter completed!