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Chapter 1436 Denuclearization

Wang Liangcai sat on the bed, staring at the envelope he received from the finance office, and was reluctant to move his eyes away for a long time.

The envelope is thick enough to make people doubt life.

JMC's paper bonus will not be available until the paper is officially published, and Wang Liangcai is still a little disappointed.

However, the prize money for the achievement award is obviously not comparable to the prize money for publishing a paper.

It was also when Wang Liang saw this envelope that he realized that the laboratory valued the results.

After all, completing a project often represents multiple heavyweight papers. If you want more papers, just read more, and they are all dependent on the results.

"One is a fairy flower in Langyuan, and the other is a beautiful jade without flaws. If you say you have no chance, you will meet him again in this life..."

The opening song of "Dream of Red Mansions" came from next door, which suddenly woke Wang Liangcai up.

The freshly released "Dream of Red Mansions" is the most attractive TV series of this year. It is not an exaggeration to say that there are so many people in the streets.

When the opening song of the TV series starts, you can hear the sound of shouting home in the yard. Adults and children who are crazy are easily called home. You can't do it if you don't go home. Friends who are playing with you will have to go home to watch "Dream of the Red Chamber", who will play with you?

There is no TV in Wang Liangcai's small suite, which is the only regret. Wang Liangcai went to the cafeteria to watch big TV two days ago, but today he was too lazy to run away.

He reached out and grabbed the envelope on the bed, poured out the brand new banknotes, counted them again, then took out most of them and put them back in the envelope, thinking: My wife has wanted to change to the color TV for a long time, otherwise, she would have used this money this year.

Wang Liangcai divided the remaining parts into three parts, preparing to leave a portion of improved food for his family, and then buy a good piece of clothes for his wife and children. Finally, buy a few good books for himself.

After the distribution was completed, Wang Liangcai was still unbelievable when he looked at the four stacks of money in front of him.

He never had a monthly income, and he was able to do four things.

Not to mention four pieces, just one quarter piece, it’s all amazing.

For example, when buying clothes, save some money every month, you can only buy clothes once when the season changes. You can’t buy them for everyone in your family. You must have bought autumn clothes for your wife this year, so you don’t have the money to buy winter clothes.

It is even more difficult to replace a battery. It is common to save one or two years to buy an electrical appliance. During this period, there are no major projects that can spend money.

So, when you go to an ordinary house and see, you can count the electrical appliances with one hand.

Wang Liangcai has no foundation at home. After working for many years, he became a professor and bought a black and white TV, as well as an electric fan, a recorder and a single-tube washing machine, which is as high as the national average. As for equipment such as refrigerators and air conditioners, it has not yet entered the discussion field.

Wang Liangcai couldn't help but touch the money with his hands, as if he was afraid that it would run away.

Turning his head, Wang Liangcai couldn't help but smile bitterly. He, who claimed to be a literati, did not expect that he loved money so much.

"A gentleman loves money and gets it well." Wang Liangcai clenched his fists and made a secret decision. He then hugged a pile of money and slept in the quilt.

Four o'clock in the morning.

After slept for 8 hours, Wang Liang got up from the bed, washed up, and went to the laboratory.

The dormitory was very close to the laboratory. When Wang Liangcai arrived at the door, he explained briefly, and the guard put him in.

Wang Liangcai rubbed his hands while walking. When he arrived at the laboratory, he felt his fingers soft. He then picked up the stove he pressed yesterday, added coal, then changed into the laboratory suit, opened the instrument cabinet, turned on the microscope, inverted phase contrast microscope, piezoelectric pulse meter, and stereomicroscope...

Super-number ovulation and egg retrieval can only be regarded as the preparation stage for cloning sheep. Next, the real challenge starts with "denucleation".

Compared with the first two operations, the latter has a stronger sense of "scientific attributes".

At least, more instruments are used.

Judging from the prices of various equipment in 1987, there are only a handful of research institutes in China that can undertake this project.

Wang Liangcai inspected the instrument and prepared the nuclear removal fluid.

There are two types of nucleation liquids that are free from sucrose and have sucrose. They can also be mixed with various strange hormones. Each time they stir into something, they can write a short paper.

Even the changes in concentration can be regarded as a small topic.

But in Yang Rui’s genetic engineering laboratory, such projects are naturally unappreciated.

Wang Liangcai just followed the traditional model and made a sucrose-free version and a 3% sucrose version. Then he took out the Petri dish, dripped it on and used it to nucleate, added cell relaxant, then covered it with paraffin oil, and finally placed the goat's oocytes in the droplets.

Just a small step in the initial stage made Wang Liangcai sweat all over, and the cold in the laboratory was no longer felt.

This is Wang Liangcai, an academician-level professor. If he were other contestants, let alone a newcomer, he would not be able to play these steps even if he was a young professor who was famous for his operations or a new professor who was just starting out.

At least, it takes one or two weeks to complete the established action 100%.

Considering that every failure here will waste one or even multiple oocytes, you can imagine the importance of experienced players.

Not to mention, the next steps are just more complex continuous actions and more test techniques.

Tong Dizhou was able to get good at Belgium back then because of his good skills.

As for why his technique is good? There is a story that specifically tells it: at that time, the people of Belgium had already started to and from get off work on time, and the researchers strictly implemented work requirements, went home to play after dinner, and Tong Dizhou stayed alone in the laboratory until two or three o'clock in the evening, and did not leave until the security guard rushed the people.

This training method was also passed down as Tong Dizhou returned to China.

"Have you ever seen the capital at four o'clock in the morning?" For genetics scholars, it is really not a problem.

If not, how can you make a reputation for suffering in the path of hard biology?

Wang Liangcai didn't feel that experimenting was hard. Compared with metaphysical physics and suicide chemistry, biology is finally normal. At least, it can improve its value through training.

The egg cells of sheep are too expensive, so you can make amphibious frog egg cells like that of Comrade Zhou.

However, it is inevitable to have a very long training and concentration.

Not only that, it is difficult for any experimenter to perfectly remember every experimental step, and it is even more impossible to know the latest research trends in the scientific research community at any time.

In this case, you still need to read a large amount of literature continuously.

Even with the help of a data group, the time saved is still limited.

Wang Liangcai also read the operating guide first, then adjusted the lens of the inverted phase contrast microscope, began to aim at the oocytes, and used a nucleation needle to gently fluctuate to find the right position.

After rubbing his hands sore, Wang Liangcai finally used the nucleation needle to cling to the cells with the help of the fixed needle and sucked the transparent belt, so that the nucleation needle maintained negative pressure. Then he turned on the piezoelectric pulse meter and tried to penetrate the transparent belt with strong pulses.

The nucleus is very small.

The cells are already small enough, but the nucleus is more compact than the cells.

It is obviously not easy to remove the nucleus from the cell.

The blind suction method that Wang Liangcai currently uses is not low, but it is easy to absorb too much substance in the egg.

The latter is an important nutrient for egg cells to develop into embryos. If the egg cells are absorbed too much, the embryo will still fail.

Wang Liangcai also knew this disadvantage, so he thought while doing experiments.

This is the case with the experiment. There is no perfect experimental method, and it is impossible not to continue because the method is imperfect.

He will think about new methods, but if there is no moment of inspiration, it is not unacceptable to use stupid methods to make results.

Unless the stupid method consumes too much resources, most of the experiments are completed by countless stupid methods, connected by several sudden inspirations.

As the sky was getting brighter and there were more people coming to the laboratory, Wang Liangcai was still selfless and continued to work.

Although he worked for four hours from four in the morning to eight in the morning, he only performed four times in terms of experiments.

And it was four failures.

For an experimental biologist who has been accustomed to self-abuse thousands of times, four times are just appetizers.

At nine o'clock in the morning, Gu Qiang came to the laboratory in a daze.

He arrived not too late, but chose to go to the cafeteria to have breakfast first and come over after he was full. However, he saw Wang Liangcai who was working hard for the first time.

"It's too early." Gu Qiang couldn't help but curl his lips, but his steps were three points faster.
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