Chapter 1444 Expert
Judging from Wang Liangcai's perspective, Yang Rui's operations are hard to say.
He was a student from the Tong family. He studied in the undergraduate degree in genetics and trained with scholars from the Institute of Aquatics of the Chinese Academy of Sciences for many years. Later, he taught and researched at the University of Science and Technology, and played various genetics projects. In terms of egg peeling technology, Wang Liangcai was a world-class, far surpassing Peking University's so-called egg peeling master, and Yang Rui's technology was even less in his eyes.
However, Wang Liangcai wanted to remember every move Yang Rui was operating.
In just a few dozen minutes - almost equivalent to watching Wong Kar-wai's movies in the cinema, Yang Rui had basically completed the denuclear operation.
Nuclear removal is not difficult, especially for researchers from aquatic products, it is even more basic.
But a player like Yang Rui failed only once and successfully completed the denuclear operation, which is the real surprise.
In other words, Yang Rui's operation method is not worth mentioning, but he easily completed the decore operation with such an operation, which is the reason why Wang Liangcai is concerned about it.
In the cloned cell operation, a total of two enucleation operations are required.
The first nucleation operation is to extract the nucleus of the sheep's oocytes. Because this nucleus is not used in the later stage, the key point of the first nucleation operation is to suck out the cytoplasm of the cells as little as possible, and at the same time, the nucleus must be sucked cleanly.
The second enucleation operation is for ordinary cells.
The cloning of sheep uses sheep's breast cells. The key point at this time is to completely suck out the cytoplasm of sheep's breast cells. As for the breast cells themselves, it doesn't matter because they are also discarded in the later stage.
In general, both steps actually require refinement, and there are few disadvantages that can be tolerated. The best state is to completely separate the nucleus from the cell itself.
Otherwise, if the nucleus left by the oocytes participates in the development of later stages, it is hard to say whose sheep they are born.
If it is used for cloning, you originally wanted to clone Dolly the sheep, but Dolly carries how beautiful the genetic gene is, so this cloning is hard to explain.
It's not a sensational clone.
From the beginning, Wang Liangcai was very clear about the purpose of this operation. However, in order to absorb the nucleus of the oocytes, he had to suck out more cytoplasm, and the remaining cytoplasm was not enough to develop, which was declared failure.
The same is true for Gu Qiang's operation. In this step, the cytoplasm is too easily sucked out.
On the contrary, the second step is that it does not matter how much cytoplasm is lost, it only requires the integrity of the nucleus, which has a much lower technical requirement for the experimenter.
Yang Rui's newly developed technology is different.
Although the operation goal has not changed, it is still necessary to aspirate all the nuclei and to aspirate the cytoplasm as little as possible.
However, Yang Rui's operation lowered a little. Not only did he not pursue a complete sucking of the nucleus, he deliberately sucked the nucleus and then sucked out the fragments bit by bit.
Although the complete aspiration of the nucleus can meet the requirements, it is too difficult.
Moreover, the cell nucleus is not a hard solid. It is more like a balloon swimming in a viscous liquid. If it is completely sucked out, it is inevitable to increase the suction force, thereby sucking out more cytoplasm. In addition, when the balloon comes out, it always brings out more cytoplasm. Sometimes it brings out more, which directly declares that the experiment failed. Sometimes it brings out less and has a considerable component of luck.
If you suck it, it will certainly cause more trouble.
Think about a balloon breaking into the liquid, and the gas inside the balloon floats out, forming bubbles in the liquid, and sucking them out one by one, how troublesome it is.
However, trouble is a troublesome job that can be done. In addition to careful and tedious operations, it is much easier to absorb fragments than to suck a whole balloon.
Sometimes, if the nuclear suction needle is well controlled, it doesn’t even need to absorb much fragments. The task will be completed after three or five negative pressure absorption.
After Wang Liang discovered this, he could no longer walk.
Immediately afterwards, Wang Liangcai discovered that sucking up the slaughter was just the smallest point in the new technology developed by Yang Rui.
The most powerful thing is to use the spindle to control the amount of absorption, and it can be said to be exquisite.
As the name suggests, a nucleus-absorbing needle is used to absorb the nucleus. How small is its needle tip? Basically, you may not be able to see it clearly under a microscope of ordinary multiples.
Therefore, when operating the nucleus suction needle, a slight tremor will cause it to swing violently.
You can imagine the feeling of holding a rope in your hand when you thread a needle. The cells are much smaller than the needle hole and tremble much more. Not to mention nuclei, it is normal to penetrate the cells.
Yang Rui's level is weaker than Wang Liangcai, and he is also an experimenter of a certain level. He still accidentally poked an oocyte, which can measure the difficulties.
However, when the nuclear suction needle pressed against the spindle, the tremor disappeared naturally.
It's like holding a long stick in your hand, the head of the stick will tremble, and when you poke the stick on the wall, the head of the stick will not tremble.
In addition to being able to control the jitter of the nuclear aspiration needle and increase accuracy, another advantage is that it controls the front and back flutter of the nuclear aspiration needle.
It means that when the nuclear suction needle pokes the spindle, it seems like it has added a fault-tolerant device out of thin air, making the operation easier.
It's easy to say, but it's not easy to use this trick.
The most difficult part is that you find the correct poke location.
The spindle is also a very important part of the cell and cannot be poked just by wanting it. It is naturally tough, but some parts are too tough, which will hinder absorption, while some parts are unexpectedly fragile and can easily poke.
The remaining spindles that can poke are preferably in a better position to operate.
Wang Liangcai looked at Yang Rui's actions and his mind was constantly moving. He wished he could master the new method immediately and try it back.
At this point, Yang Rui could not meet Wang Liangcai's requirements.
He did it slowly, and he only tried a few new methods. His operation was so clumsy that it made people want to jump, just like a child who had just learned to shoot a ball and made a three-step layup. He was ugly when he didn't foul, let alone more times when he fouled than when he walked.
"Professor Yang, I understand a little bit, otherwise, I'll try it?" Wang Liangcai said in a low voice.
Yang Rui raised his eyelids and said, "What do you think my operation is not good?"
Wang Liangcai coughed twice and said against his will: "How can you do it? You have no problem with your operation."
"I also think, wait a moment, I will complete this decore." Yang Rui did feel that he did a good job, not as good as Wang Liangcai's operations, and has made progress compared to previous operations.
Naturally, he ignored the difficulty gap between old and new technologies.
Wang Liangcai was so anxious that he couldn't urge him, so he could only watch obediently.
If he had added two kilograms of bear cell nuclei to Wang Liangcai, he would have jumped up long ago to tell the truth. The same conditions would also apply to other researchers in the laboratory.
As time went by, Su Fan, Gu Qiang, Tian Bing and others all gathered silently to the experimental table.
Everyone knows how Yang Rui's experimental ability is.
Everyone knows how long an oocyte can withstand the ravages.
Normally, if you give Yang Rui an oocyte, he can't play for two hours without exposing it.
No matter how long it takes, the experiment will be more advanced.
Thinking about Gu Qiang's luck was so lucky that he could take the nuclear power, and Wang Liangcai slept in the laboratory for several weeks before he could barely catch up with the progress... Yang Rui couldn't take Gu Qiang's luck, and he had to do it for several hours in one breath.
The more researchers you pay attention to, the more curious the other researchers will become.
In the afternoon, the experimental bench where Yang Rui was located was already surrounded by people.
Yang Rui had no idea.
It's not like the boring way other people do experiments, he's trying new methods, it's a bit like playing a new game, and he's always unable to stop at the beginning.
Gudu.
Someone's stomach rang.
Yang Rui raised his head blankly and rubbed his sore neck, only then did he notice all kinds of strange gazes around him and the moving heads.
"Hey, don't squeeze it all, aren't you guys?" Yang Rui helplessly drove everyone away and said, "Be careful, don't pollute the laboratory bench."
Everyone quickly took two steps back and gave up a piece of space.
"It's almost eight o'clock, so those who haven't eaten should go to eat. It's still a few hours." Yang Rui touched his stomach and decided to wait and eat. His stomach could wait, but the cells could not wait.
Everyone nodded and persuaded each other: "Laotian, you have a bad stomach, hurry up and have a meal."
"Xiao Lei, don't be stuck, go and have a meal."
"Su Fan, today I have the braised pork you like, your hands are fast and your hands are slow and you don't have any."
"Then why don't you go?" Su Fan curled his lips and didn't care about the persuasion of others.
In fact, no one wants to leave at all.
How rare is the opportunity to watch new technology demonstrations? Yang Rui, who is a Nobel Prize-level, was presenting it. He said later, "When I saw Professor Yang do egg cell nucleation," he naturally became very stylish.
Yang Rui didn't say much. He was just demonstrating the technology and couldn't drive people away.
He touched his stomach and continued to fuck, and the others gathered together as usual, and discussed in a low voice while watching.
There are always differences in a laboratory level, especially when facing a specific topic, some people always have a thorough understanding of it, and some people are always not very good at it.
At this time, everyone's attention is different.
The so-called laymen watch the excitement, while the experts watch the tricks, and the same is true for being in the same laboratory.
Chapter completed!