Yang Rui took out a Montblanc from the drawer, patted his head before putting it down, and said, "You go and copy one first, so that I won't mess it up."

"It's okay, you can just take a slap." Gu Qiang's attitude was extremely well-behaved.

"It's better to copy one and leave a foundation, so you can compare it later." Yang Rui insisted.

Gu Qiang had to copy as he said, looking at the hundreds of pages of experimental design, and was a little reluctant to give up. It was not that he couldn't give up on the experimental design he wrote, but that he couldn't give up on the copying of paper and ink.

Today's photocopiers are all Japanese products. Not to mention the price of the machine, the consumables are even more expensive. When Yang Rui was working on the exam papers at school, he organized mimeographs.

For ordinary materials, everyone uses copying methods, and try not to waste consumables such as copy paper.

At this moment, I naturally couldn't wait to copy it.

Gu Qiang toughened his teeth and printed a copy of the experimental design he wrote. When he returned to Yang Rui, he said: "From the future, I will find someone to copy these things. It will be too expensive to print, and the paper costs a few cents a piece of paper."

“It’s not expensive to find someone?”

"For students who work and study, 10 yuan a month is enough." Gu Qiang said for granted.

10 yuan is a lot. Nowadays, rural families in many places have less than 120 yuan in cash income per year.

If students eat in the cafeteria, 10 yuan can ensure fullness and also bring some meat.

Yang Rui ignored this, looked down at Gu Qiang's experimental design, and took off his cap while saying, "You have to save some office funds. Leave it to Director Xu to worry about it. Don't waste your time on this. The laboratory wants you to produce, not to save money."

"What is saving is output." Gu Qiang whispered.

"If you can complete the topic of in vitro cultivation within two months, it will be the real output." Yang Rui's red pen drew over Gu Qiang's experimental design, and the first line circled three circles.

Gu Qiang was shocked by the red mark, but also shocked by what Yang Rui said, and couldn't help but repeat: "Two months?"

"The progress of Wang Liangcai's research team will also accelerate." Yang Rui didn't say why, everything was indifferent.

Gu Qiang's mouth twitched twice, knowing that Yang Rui must have intervened in Wang Liangcai's research.

Think about it, it is impossible for Yang Rui not to intervene.

From the beginning of the cloning project to now, Wang Liangcai and Gu Qiang's team have been busy competing and doing experiments, and Yang Rui has never stopped thinking.

Now, he will naturally help improve the progress of the project by expressing his considerations.

However, in two months, the topic of in vitro cultivation would be developed from scratch, but Gu Qiang was not confident.

"I originally thought that time would be more affordable." Gu Qiang said cowardly.

Yang Rui smiled, picked up the pen again, and began to draw a red circle.

On the first page alone, he drew more than a dozen red circles, almost beyond recognition.

Gu Qiang took a breath, then lowered his head and looked at the place Yang Rui cut out.

The term "in vitro fertilization embryo" was enclosed and a forked one. The oxygen consumption next to it was enclosed without any other modifications.

Later, Gu Qiang saw the circle drawn by Yang Rui for the word "densification" and the circle drawn by "culture medium".

Gu Qiang finally knew why Yang Rui asked him to copy one.

After this modification, the entire experimental design will be beyond recognition.

If you don't leave a base plate, you really don't know the original specific content.

"I hope you can focus on embryos after blastocyst phase for research. However, the key points you need to pay attention to are not only that." Yang Rui saw that Gu Qiang was watching, and did not seem to have suffered too much damage, so he explained while drawing circles and forks, and said: "If you want to refer to the culture system of in vitro fertilization embryos, I don't agree with it."

Yang Rui clicked the pen twice on the red cross and said, "Of course, everything is the first criterion for the judgment of the researchers on the front line, but from my personal understanding, if you don't do analysis, you will directly adopt the original system. The result is probably not to save time, but to waste energy."

"Do you think cloning embryos will be different from normal embryos?" Gu Qiang couldn't help but be surprised.

Without talking about theological things, scientists have a clear understanding of genital bodies such as embryos and have a clear understanding of cloned embryos, believing that cloned embryos should be no different from normal embryos. Otherwise, why would cloning cause so much controversy?

Therefore, during the in vitro culture, Gu Qiang naturally adopted the same technical solution as "in vitro fertilization" and "in vitro cow".

Yang Rui put the pen aside and said, "I don't think cloned embryos will be essentially different from normal embryos, but of course they are different. First, the cloned embryos we obtained are developed with egg cells with very few cytoplasm and are inherently bad. Secondly, the oocytes have been soaked in a variety of operating fluids, such as what we know soaking in sucrose solution will have adverse effects..."

Yang Rui's voice was steady and said, "In short, cloning embryos is an abnormal embryo. If you use normal embryo culture methods, there may be problems."

He said it was a guess, but in fact, Yang Rui was sure it would have problems.

This is also a difficulty in in vitro culture.

More problems will arise with undeveloped oocytes.

In short, in order to change the nucleus of egg cells, the scientists' operations have caused great harm to them, and in vitro culture is to make up for this damage.

For example, if you regard 5 kilograms as the minimum standard weight of a newborn, it is very difficult to survive at 2.7 Newton at birth. And the modified cloned oocytes, which are about 0.5 kilograms, will never survive under natural conditions.

To make it develop normally, what it faces is no longer the problem of whether it is fed Sanlu milk or whether it can afford imported milk powder.

Of course, if you can't afford to buy imported reagents, the oocytes will not be able to survive.

Gu Qiang was shocked by Yang Rui's detailed explanation and couldn't help but ask in a low voice: "Have you done an in vitro cultivation project before?"

"When we were doing some preliminary research when we were doing embryo transfers for cows," said Yang Rui.

Gu Qiang was full of suspicion that although the embryo transfer of cows also carries the word embryo, it is orders of magnitude different from the problem we are facing now.

Not to mention the other side, the amount of money for in vitro cultivation of cloned sheep will cost as many as millions. The resources left before Gu Qiang's group must be transferred.

What about cattle embryo transfer? With the hidden investments of companies such as China Animal Husbandry, there is not so much in total.

Gu Qiang licked his lips and asked, "When you do a cow embryo transfer, you want to do cloning."

Yang Rui smiled and looked at Gu Qiang without answering. He used a pen to design the experiment and said, "Next, there are the details of the experiment. Do I have some ideas to provide you with reference?"

"You said." Gu Qiang's attitude was positive.

"From the data point of view, adding various additives to the basic nutrient solution should also improve the development of cloned embryos." Yang Rui did not elaborate on the information, and said: "We started culturing cell embryos, and we had to face embryo development blockade."

Embryo development blockade refers to the phenomenon that the embryo develops to the four-cell stage and stops its development. This is mainly related to the activation of the embryo genome.

There is no genome plan now, and to break this block, many various attempts have to be made.

Yang Rui didn't say it in detail, but just drew two circles in Gu Qiang's experimental design and said: "I agree with these two plans. You can try it first."

"Okay." Gu Qiang nodded repeatedly.

"The culture medium is also a key point. You must keep a good experimental record and complete experimental record." Yang Rui specially reminded.

A clear formula is very important for the culture medium.

Of course, scientists today are not aware of this, because no one has started doing cloning.

In later generations, if cloning is the most complex and most difficult part, it is not the technique of peeling eggs, but the preparation of embryo culture medium.

Yang Rui thought about it and decided to help Gu Qiang reduce the burden a little more, then said: "From my personal feeling, I think the more promising additives of the culture medium, fetal bovine serum, have been used before. Epidermal growth factor, this is a new thing, and I should try it. Insulin growth factor, antioxidants, anthocyanins, I think you can consider them. In addition, oxygen is also very important."
Chapter completed!