Chapter 454 Advanced Hot Spring Room
Yang Rui stayed in the sanatorium and set up the equipment with Liu Shan.
There are many ways to isolate strains. In 1879, the French isolated high-temperature strains from the Seine. After more than 100 years of development, the treatment methods of samples have long been diverse. The difference between the times is more about simplifying methods and improving yield rates.
Of course, the cost also increases accordingly.
Yang Rui first prepared a centrifuge - a machine commonly used in biological laboratories that cannot be used anymore, for centrifuging water samples and mud samples.
Under the action of a centrifuge of 10,000 times per minute, the water sample will directly separate the impurities enriched with bacteria and supernatant.
The isolated bacteria are of relatively large mass, and the supernatant will also be eluted from the bacteria through the filter membrane.
They are then cultured in culture medium and can be screened.
The principle of mud samples is similar, but you need to suspend the spring water first, then centrifuge at low speed to remove large particles, and then use water samples to treat and enrich bacteria.
The whole process, theoretically speaking, is very simple, but the equipment is not simple.
A centrifuge that can be transferred 10,000 times per minute is not cheap, and its capacity is smaller. It takes ten minutes to rotate one test tube at a time, and it takes hundreds of kilograms of samples to be processed. I don’t know how long it takes.
The culture stage is also very expensive. The agar, glucose, peptone, etc. used in the culture medium are much more expensive than foods of the same weight.
In addition, the cultivation conditions of 70 degrees or even above 80 degrees also put huge pressure on the cultivation cost.
Kang Hong helped Yang Rui get the equipment and materials. When he opened it, he saw that the only little flame of competition was extinguished.
He didn't want to compete, and isolating strains is not a new technology. The only meaningful thing is that it can be isolated.
If you isolate a powerful strain, you can naturally invent awesome papers, but if you are isolated only ordinary strains, it is difficult to enter the SCI journal.
The results were as vague as if they were buying lottery tickets, but Yang Rui's investment cost was so high. Kang Hong felt that he lacked competitive value when he saw it.
"Is it worth spending so much money to isolate thermophilic bacteria?" Kang Hong asked when he thought of it.
"The instruments are not bad, and after we increase our manpower, these instruments will still work." Yang Rui answered while plugging in and sorting the wires.
Kang Hong picked up a large box of filter membranes, put them in the corner, and said, "What about consumables? These are ultrafiltration membranes, all imported, so many of them need to be used?"
Yang Rui said with a "happy" and said, "I originally planned to deal with one batch first and then take one batch back. These costs cannot be saved."
"It's too expensive. We can't afford it." Kang Hong tsk twice.
"If you are lucky, there will probably be some left." Yang Rui said casually, and Liu Shan assembled the incubator.
There are many types of filter membranes. What ordinary people are more familiar with should be the filter element in a home water purifier, which is also a filter membrane. In the early stages, these civilian products all came from laboratories, and were naturally valuable in the 1980s.
In fact, laboratories have higher requirements for materials and are never cheap at any time.
In order to isolate the thermophilic bacteria you want as soon as possible, Yang Rui was very willing to spend money. Kang Hong had no reason to make such a large investment, so naturally he could not understand it. He could only attribute it to the people in Beijing University who are stupid and have a lot of money.
Yang Rui had no choice but to do so.
Isolating strains is a relatively common research job, but isolating the desired strain is not the case.
Historically, most antibiotics have been separated from soil strains. For twenty or thirty years, biologists have almost ruined soil around the world. The most successful one was Waxman, the terminator of tuberculosis, who invented a systematic separation method. He then obtained streptomycin and then won the Nobel Prize.
Waxman organized up to 50 research dogs for this purpose, and spent several years screening out more than 10,000 strains. He got the one he wanted...
Yang Rui couldn't afford to raise 50 experimental dogs, and he didn't have a few years to waste it. Fortunately, he knew which hot spring it was. As long as there were enough separations, he could always find the breed he wanted.
A simple laboratory was installed in half a day.
Yang Rui casually grabbed a handful of soil from the ground, mixed some liquid, put it into a centrifuge, and started to inspect it.
The centrifuge with ten thousand revolutions had a lot of noise, but it was so loud that Kang Hong was full of joy.
"We also have one in the yard, which is locked with a wooden cabinet." Kang Hong stared at the machine in the transparent glass and suddenly said.
Yang Rui understood: "This is true for the school's public laboratories."
"So is Peking University?" The information is now behind. The distance from Yunnan to Beijing is even further from Beijing to Los Angeles, and it is almost as fast as Fuzhou to Taipei.
Yang Rui recalled and said, "It's almost the same. The administrator of the public laboratory should be as long as the instrument is good. He doesn't care whether it's convenient for you or not."
"That is, we have to apply for a good machine at least two months in advance. If we don't do it well, we have to treat you. After that, people say that we have issued a false invoice and all the invoices will be given to these grandsons." Any researcher, when it comes to the use of instruments, was full of fire.
When Yang Rui was a graduate student, he was also afraid of using public laboratory instruments. Of course, what he feared most was to borrow other laboratory instruments. It was not easy to see others' eyes, and it was even worse to rub the instruments under other people's noses.
Anyone who has been in the laboratory for half a year has a deep understanding of this kind of pain. Yang Rui tried every means to make money and build his own laboratory, and half of the reason was to avoid the recurrence of the dilemma.
If it was just to satisfy personal expenses, how could he have such trouble? He bought a monkey ticket worth 2,000 yuan, and then threw it away and changed his house, and casually became a 21st century billionaire.
However, it is just a billionaire, but it is not interesting to decide the research direction and conduct experiments by yourself.
If there is no tremendous pressure and personnel struggle, as the person in charge of a laboratory, do the scientific research you want and do it, it will be quite fulfilling.
Kang Hong did not have Yang Rui's experience, nor did he know how to get rid of the current restrictions. He just asked enviously: "Then where are the instruments you brought out? Isn't it provided by Peking University?"
"It should be considered funded by a Hong Kong company."
"Hong Kong people are really rich." Kang Hong asked: "Where did you know them?"
"I wrote the paper, and they are interested... Well, the centrifuge stopped, it looks good." Yang Rui took this opportunity to leave and became busy.
Under the bright moonlight, Liu Shan was stretching her waist and taking off her top.
In the darkness, Yang Rui saw beautiful curves and heard tiny sounds in his ears.
…(To be continued. If you like this work, you are welcome to Qidian to vote for recommendations, monthly votes, and your support are my greatest motivation. Mobile phone users please go to m to read.)
Chapter completed!