Yang Rui slept in the ion channel laboratory for several days in a row.

It wasn't that he wanted to overdraw his physical strength from the beginning to fight, but because of the high fighting spirit of Comrade Su Xiankai, Fan Zhenlong and Wang Sisheng, he had to work overtime.

In fact, if you enter the middle of the experiment, the three of you working overtime may not necessarily affect him. The scientific research ape is exhausted and the breeder travels around the world to give speeches. However, the early stage of the experiment is different. There are too many things that require Yang Rui to confirm, so that the three of you have too many problems so that Yang Rui can't hide.

This is the job of the project leader. If the big cattle and calf under his command have no problems in scientific research and dogs and are working step by step, then he can work step by step, or even take a vacation step by step, just like a rancher who can enjoy the fresh air, the delicious steak, and the plumpness of cowboys or the plump cowboys or sheep in the weather, the weather is good, the cowboys and the surroundings are all in trouble.

However, the weather is good, the cowboys are good, and there are too few things around.

Especially a newly built ranch was a matter of flocking in. Just determining to purchase reagents was a busy afternoon.

If the G-protein coupled receptor group is smooth, Yang Rui can never ask about it if he buys reagents. It is just a routine laboratory life, just like buying a brand of gloves and which type of paper draw is as easy as buying.

But at the beginning, Yang Rui really couldn't take these small problems lightly.

The simplest reagent purchase also requires compatibility. For example, buying domestic reagents or imported reagents seems to be very easy to say. If you have money, you can buy imported ones. If you don’t have money, you can buy domestic ones.

For scientific research dogs who graduated in two or three years, perhaps the answer is true, but from the perspective of the project leader, the problem is completely different.

What Yang Rui wants to determine at the beginning is actually the reagents needed for the entire experimental cycle. Otherwise, if you use imported reagents in the early stage, domestic reagents in the later stage, and then compare the two, the result curve will definitely be messy. In this high-standard international scientific research competition, H articles are all aimed at CNS, and the pictures in them will be read repeatedly or even repeated. A mess will ruin a good data.

Furthermore, purchasing reagents is not limited to the difference between importing and domestic products. Yang Rui’s final answer on paper is not only to decide which country it belongs to, but also to which company it belongs to which factory it belongs to, or even a certain batch of it belongs to.

The purity of reagents can be selected from the best grade, analytical purity, chemical purity, chromatographic purity, etc., which correspond to the number of impurities. However, for some reagents, no matter how high the standard is, it is still impossible to completely eliminate impurities. For those who do experiments, this is a very annoying thing. If you don’t have the conditions, how can I do the experiment?

Unfortunately, it does not mean that if you do not meet the actual conditions, you can make scientific research. The correct way is to ensure the consistency of impurities. Impurities in the same batch of reagents in the same factory can usually be guaranteed to be consistent. In this way, through comparison and calculation, the influence of impurities can be largely eliminated.

If you extend the same method, you can draw two conclusions. First, even high-purity experimental reagents are better to buy a batch, because you don’t know if the other factory will produce a batch of unqualified reagents through brain excitation, which will make you encounter. Putting your own years and millions of dollars in the care and professionalism of others is not cautious or unprofessional to yourself.

Not to mention, scientific research is originally doing things with low probability.

The second conclusion is that you can use reagents of lower purity to do relatively high-demand research. As long as the data is processed later, this is a process that completely conforms to the rules, and the results are similar to those of experiments made by reagents of medium and high purity. Chinese laboratories have always done this, and the main reason is...of course, it is because they are poor.

In addition, the purer the reagents are not bought, the better.

The first question I face is naturally whether the purse can bear it. If the purse can bear it - in extreme cases, there are actually examples of not buying high-purity reagents. For example, those who make materials often like to buy domestic reagents, because domestic reagents often have inexplicable impurities, which can occasionally bring surprises to people.

There are many examples of the so-called impurities making meritorious contributions in the history of science, and they became popular in the late 6th and early 2017.

Secondly, the purse is not just about buying reagents and just looking at the expensive ones.

You can't buy a pure-analyzed sulfuric acid used to treat waste liquid. Even if you have rich oil, it's a bit too hard to disassemble a pure-analyzed bottle and fill it with a pure-analyzed bottle.

It's totally thankless.

Besides, there are no one who does scientific research on the earth who is really rich. Take the biological laboratory in 1986 as an example. A gram of interferon of 50 million US dollars, just say how many tons you want.

G-protein-coupled receptors are originally a money-burning project. The start of $6 million is enough, but they must not be wasted at will, especially in the early stages. The standards must be set more carefully.

You can't just come to a double base immediately because the fog of war has opened.

Many of the reagents used in the early stage have to be used all the way until the project is over. If the project is to be carried out for three years, five years, or even five years, it is impossible to suddenly change to cheap reagents in the fourth and seventh years, then the value of the previous experimental data will be greatly reduced. Therefore, instead of gritting your teeth at that time, you must establish your own standards in the early stage.

This is actually a challenge for Yang Rui.

There is no one to teach you how to plan the beginning, and there is no fixed number. The world is changing too fast, and to some extent, there are some predictions. This kind of prediction not only requires predicting the time for the project and the expected funding, but also predicting the status of the opponent and the trend of the scientific research community.

Of course, the most important thing is experience.

If you are in developed countries such as Europe and the United States, Yang Rui can try to ask scholars from other top laboratories. Unfortunately, there are no corresponding conditions in China.

It is Professor Cai and others who have made international results, but they have not formed international laboratories and have obtained international results while carrying out international projects.

In short, China has not had such targeted large-scale biological research and development since artificially synthesized bovine insulin. In the past 20 or 30 years, there have been basically no scientific research in biology, ready for war, and then participating in frontal international scientific research competition. In terms of this, it is almost all ambush war special wars or special agent teams behind enemy lines.

Looking back to the past, when the Institute of Biochemistry, the Chinese Academy of Sciences, the Department of Chemistry of Peking University and the Institute of Organic Chemistry of the Chinese Academy of Sciences gathered heavy troops to organize the dispatch of windbreakers and lead the pre-war mobilization. Each of them had a scalpel and started to synthesize bovine insulin by artificially, "Science" and "nature" were published one after another. Although the whole country was fighting against only an American laboratory and a German laboratory, in specific scientific research fields, this was indeed the highest level of confrontation. To say that shocking the world may not be possible, shocking China was indeed done.

However, that was also the past 30 years ago.

So, Yang Rui had no place to ask for advice.

However, from a positive perspective, this also left Yang Rui without competitors and less expert guidance from pointing fingers.

"Anyway, I spent my own money, I paid my tuition fee..." Yang Rui gritted his teeth and signed various documents and projects.

The dollar account of the People's Bank of China has also declined rapidly. The business managers of the distressed branch called to inquire.

$100,000.

$200,000.

$500,000.

$1 million.

$2 million.

By the time the preparations were completed and the order of the instruments and consumables to be used in the early stage was completed, the $2.7 million had already been out.

Looking at the numbers released inside the laboratory, Su Xiankai felt bored at that time, and suddenly felt a cold sweat on his back.

"Fortunately you didn't listen to me at that time." Su Xiankai followed from beginning to end. He knew that Yang Rui did not choose particularly expensive products. He basically searched for them within the medium-sized range. It was a relatively frugal film opening model. It was not that he could not be frugal anymore, but if he continued to be frugal, he could only do tracking experiments and was not qualified to participate in scientific research competition.

However, just by choosing this standard, it actually reached 2.7 million US dollars, which was almost three times the number Su Xiankai wanted, and Su Xiankai was also scared.

There is no turning back when the start of construction, and the $1 million has been spent. If you don’t see the noise, you will not be able to recover it.

Yang Rui didn't feel grateful, and said: "No need to say that success is successful once. The remaining funds can make people die of death, and maybe one or two seven-digit numbers are left..."

"Director Yang, don't provoke us. We haven't even found a door." Fan Zhenlong couldn't even laugh. Recently, just a purified G protein was used to train him and Wang Sisheng, let alone the whole experiment.

Su Xiankai, a skilled worker who has purified a group of G proteins, was not confident at all and said: "If you do rhodopsin protein, then there is another success. I already feel it here, but your requirements must not be so low."

Yang Rui flipped his eyelids and said, "Boy rhodopsin protein is an experimental material, and it has not entered the experimental steps yet. Lao Fan, where are you here? Is the detergent easy to use?"

Using detergents can increase the solubility of G protein, which is currently the best way to purify G protein. It is also a secret in high-end laboratories and has not been widely spread.

But Fan Zhenlong still shook his head and said, "It's easier to use, but we haven't found the trick to pull the receptor out of the cell membrane. Lao Su doesn't understand."

Yang Rui naturally looked at Su Xiankai.

Su Xiankai was embarrassed and said, "Lao Fan and the others chose to purify Gt, and I have never been exposed to it. I can't grasp all the doses correctly. I actually have no tricks, I just did it a lot. It took me a year to purify it."

There are many types of G protein and G protein-coupled receptors, and a certain amount must be used to make a systematic comparison. Therefore, although purification is for Fan Zhenlong and others to practice, it is also one of the necessary conditions in the early stage of the experiment.

But it would be hard to wait for another year or two before a purified protein was released, and the laboratory could not afford it.

Yang Rui couldn't help but lower his head to ponder.

He also saw this secret to purifying G protein with detergents, and he had not done it on the spot. Moreover, as far as his experimental level is concerned, there is no specific experimental guidance.

In any case, the experimental level of the Mavericks such as Fan Zhenlong is better than his. However, if they can become big bulls in the future, they do not rely on their experimental level. In terms of experimental level alone, the three of them are afraid that Gu Qiang can still play.

Thinking of this, Yang Rui couldn't help but pat his forehead and said, "In this way, I'll add another farmer to you... No, add an experimental assistant, Gu Qiang from the Genetic Engineering Laboratory, has a strong hands-on ability, you can cooperate with each other."

Learning also requires assistance. Since the idle mining speed is slow, add a farmer.

Anyway, the genetic engineering laboratory is also under his name and is also public laboratories, so it is very convenient to second and other things.
Chapter completed!