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Chapter 455 Simple experiment

In the small courtyard, the ground paved with bluestone bricks is a pool of hot spring water in the middle, which steams a little gas.

The moonlight was shattered and fell on the hot spring water, as if a faint blue color rose.

Liu Shan seemed to slide and fell gently into the water, like an elf in the night.

Her slender waist and proud hips drip out a curve in the air, and she was impressed as if time was still.

Yang Rui was holding a bunch of things in his hand, a little hesitant.

"Give me the egg." Liu Shan held up half of her body and waved from the hot spring.

"Oh." Yang Rui took two steps and handed the egg basket to Liu Shan.

At this time, he saw Liu Shan's all-black one-piece swimsuit and rouge cheeks, and he didn't know if it was the hot spring.

"I'll make hot spring eggs, you're responsible for cooking instant noodles." Liu Shan slashed her feet twice and placed the eggs under her feet.

Yang Rui looked around and hesitated to go where to cook noodles.

Hot spring water can cook hot spring eggs, but it can't be used to cook hot spring instant noodles.

Liu Shan looked at him and suddenly felt a little funny. He pointed at the back door and said, "It's tied there, you can go and bring water over."

Yang Rui suddenly realized and went to the back door in a daze. It was indeed a latch without a lock.

When you open the back door, the street lights on the road are extremely dim and there are no people or animals. Most of the sanatoriums are veteran cadres, and the most lively time every day is ninety o'clock, and after dinner, one of them goes to bed earlier than the other.

Although there are not many people like Yang Rui who come to play with him, there are still not many. Yang Rui gently closed the back door, returned to the room in three or two steps, and brought back the boiling water and a bowl. The hot spring eggs were just cooked.

"Eat while it's hot, I'll cook the noodles." Liu Shan soaked in the hot spring for a while and felt tired. She leaned against the edge of the pool, tore open the instant noodles, and started to put seasonings.

Yang Rui could also see his enchanting figure underwater with his light.

Although I have seen countless pictures and influences, I feel that it is extremely different when I see real beauties, real beauties in swimsuits, especially when they are alone.

Yang Rui felt that his body was extremely active, and the elm all over his body seemed to be alive.

"I'm going into the water, too." Yang Rui took off his shirt in a few seconds, then wore shorts and slipped out of the hot spring.

Liu Shan turned her back to him, and she couldn't tell her expression, but her movements were stiff for a moment.

A few minutes later, the instant noodles were soaked, and the two of them lay down by the hot spring and started eating side by side.

The water flows warmly, and the eggs cooked in the hot spring are not ripe yet, and they are extremely tender.

Yang Rui was sure that this was the most delicious instant noodles he had ever eaten.

...

It was the third day that Wei Zhenxue and others returned to the sanatorium.

The mountain roads in Tengchong area are much harder to walk than they expected, and several people suffered some skin trauma to some extent. In addition, it was also very difficult to carry nearly 100 kilograms of samples separately.

As the youngest experimental dog, Sun Ruyue played a huge role. He was like a husky dog. He came back with more than 30 kilograms of things alone, and said shyly: "I am used to doing farm work in my hometown."

"Eat a hot spring egg." Yang Rui patted Sun Ruyue on the shoulder, then raised his thumbs up and praised: "It's done well."

Sun Ruyue's fatigue from being praised disappeared and she instantly smiled from the Eskimo dog to the Samoyed, saying, "This is what I should do."

"Stay good rest for the night, and I'll be busy tomorrow." Yang Rui used to be an experimenter. If he followed his tutor, he would have to do the chores of taking samples.

Turning his head, Yang Rui began to check the samples.

When the sample is in a hot spring, it is just ordinary mud, ordinary spring water, and ordinary soil. But when it becomes a sample, the word "holy" should be preceded by its name.

The sample is like a vassal nun, and from the moment it is put into the box, it will no longer allow others to be defiled until the owner of the box, such as a bishop or researcher, open it.

"I'll do a few sets of tests now. You can rest first and help tomorrow." Yang Rui couldn't help but start. Although the sample was properly stored, any storage device could not withstand time, and the best storage method was always used as soon as possible.

At the very least, Yang Rui had to make sure that these samples were not contaminated and that thermophilic bacteria were active. Otherwise, if you return to the capital and find that the samples were not enough, it would be troublesome.

The isolation of thermophilic bacteria is only the first small step among countless steps. Thinking about the distance from PCR, Yang Rui slowly felt a sense of urgency.

Although in later generations, countless people evaluated PCR technology without surprise - it is true that this technology that can replicate infinitely is more like a delicate technological convergence than a technological innovation, technology without surprise does not mean that it is easy.

It takes half a year for graduates from the biology department to do a graduation thesis. How many people copied the experiments in the book and couldn't do it no matter how they were done. Then they burst into tears like a female driver staring at the parking space on the side...

The application of PCR technology is so widespread that it is destined to be exquisite in its design.

The dinosaurs in Jurassic Park were cloned using PCR technology. The so-called "just a little blood" can be measured. It does not mean that there is very little blood required for DNA measurement. In fact, there is PCR technology that infinitely replicates the DNA contained in a little blood, which makes it easier to test.

Before PCR technology, in other words, DNA replication was a very troublesome thing in 1984.

PCR technology is revolutionary in application.

The original author Muris spent more than three years on such a revolutionary achievement. He was a lazy person, but sometimes he was quite diligent, but that was without competition.

What would he think if he knew that Yang Rui had similar research?

Will it speed up?

Or give up.

Yang Rui didn't know that he had to prepare for the worst.

As for not doing PCR, don’t intercept it. Yang Rui would not consider such a choice.

If we look at the personal interests, if we don’t implement the Nobel Prize-related technology, then what is that?

If we take the moral high ground, PCR one or two years ahead of schedule will bring about the miracle of countless lives. Biology is not a vulgar technique of farming, mining or machinery. It is a discipline directly related to life. Implementing an important biology technology a year earlier means that countless new drugs may be invented one year earlier, which means that countless patients may be saved one year earlier, which means that countless people's lives may undergo earth-shaking changes...

Of course, Yang Rui never demands others or his own with a high degree of morality.

However, he felt more or less "being obedient in heaven" and when he was doing serious things, his spirit was extremely focused.

Liu Shan looked at Yang Rui quietly for a while from a distance of several meters, then smiled, rolled up her sleeves, and joined the ranks of experimental assistants.

Wei Zhenxue saw it and stopped resting and said, "We will help too."

"You go to rest, so are Xiao Sun. They all go to rest. I'll be busy tomorrow." Yang Rui shook his hand, lowered his head to take the sample, and said, "I'm not polite in the laboratory, you all go to rest. I'll do a few sets of experiments to try."

Wei Zhenxue hesitated for a moment and looked around the blue brick walls, unable to be sure whether this place is considered a laboratory.

However, it must be calculated after all.

Three hours later, Yang Rui found the first thermophilic bacteria.

Although I don’t know if it’s the variety I want, this at least proves that there is no problem with the sample.

Yang Rui felt relieved and immediately went back to his room to sleep.

You must have plenty of energy to complete tomorrow's experiment.

The biggest difference between doing experiments and doing handicraft classes is that thinking in experiments is essential.

Handicraft classes are the steps designed by others, and at most they are to do it in the middle. When you are uncertain, ask: "Hey, where did I just stick to?"

There are many questions about doing experiments, especially exploratory experiments, where all the experiences of predecessors can only be used as reference.

For example, the thermophilic bacteria in hot springs. If they are archaeas from the dinosaur era, then studying them is almost the same as the research steps after a time shuttle arrived at the dinosaur era and scooped up a pool of water.

In fact, studying thermophilic bacteria in hot springs is a bit more complicated because it is not yet certain that they do come from the dinosaur era.

To do such an exploratory experiment, thinking at any time is necessary in the experiment, and thinking at any time means brain-burning at any time.

In the next few days, everyone in Huarui Laboratory spent the brain-burning.

Yang Rui gets up every day and eats breakfast, he starts playing with the centrifuge. In addition to taking a hot spring in the afternoon, he completely requires himself to meet laboratory standards.

Kang Hong came here and saw that their working conditions were so nervous, so he was embarrassed to stay for a long time. He stayed back to Kunming.

Yang Rui actually didn't notice how long he stayed. His head was full of the speed of the centrifuge and the temperature of the culture medium.

The treated substances obtained from the centrifuge are enriched bacteria, and there is no idea how many of them are in a tube. These bacteria are directly applied to solid culture medium and placed in incubators at 80 degrees and 85 degrees respectively.

Ordinary bacteria that cannot survive in this environment will naturally die. They can survive and grow into colonies. They must be cultured separately, and finally single colonies are selected to cultivate them to achieve the isolation and purification of the strain.

In short, what Yang Rui and the others got were bacterial great-grandchildren. The bacteria themselves just rolled on the culture medium and exerted their reproductive ability, and then they passed away with the wind.

In this process, a lot of resources are consumed.

Bacterial culture medium is more expensive than pure broth!

The culture medium on each plate is full of the strong smell of banknotes.
Chapter completed!
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